PUBLICATIONS

Davis, J.L., Norwood, J.S., Smith, R.E., O'Dea, F., Chellappa, K., Rowe, M.L., Williamson, M.P., Stafford, G.P., Vinogradov, E., Maes, E., Guérardel, Y., Mesnage, S.

Dissecting the Enterococcal Polysaccharide Antigen (EPA) structure to explore innate immune evasion and phage specificity

(2025) Carbohydrate Polymers, 347, art. no. 122686, .

DOI: 10.1016/j.carbpol.2024.122686

Rivu, S., Smith, E., Stafford, G., Ahmed, S.

Draft genome sequence of Listeria aquatica strain SG_BD1, isolated from a cow dung sample in Bangladesh

(2024) Microbiology Resource Announcements, 13 (9), .

DOI: 10.1128/mra.00729-24

Alrafaie, A.M., Pyrzanowska, K., Smith, E.M., Partridge, D.G., Rafferty, J., Mesnage, S., Shepherd, J., Stafford, G.P.

A diverse set of Enterococcus-infecting phage provides insight into phage host-range determinants

(2024) Virus Research, 347, art. no. 199426, .

DOI: 10.1016/j.virusres.2024.199426

Alrafaie, A.M., Stafford, G.P.

Enterococcal bacteriophage: A survey of the tail associated lysin landscape

(2023) Virus Research, 327, art. no. 199073, .

DOI: 10.1016/j.virusres.2023.199073

Gains, A.F., Lambert, D.W., Stafford, G.P.

Identification of a Czc-like operon of the periodontal pathobiont P. gingivalis involved in metal ion efflux

(2023) Anaerobe, 80, art. no. 102696, .

DOI: 10.1016/j.anaerobe.2023.102696

Satur, M.J., Urbanowicz, P.A., Spencer, D.I.R., Rafferty, J., Stafford, G.P.

Structural and functional characterisation of a stable, broad-specificity multimeric sialidase from the oral pathogen Tannerella forsythia

(2022) Biochemical Journal, 479 (17), pp. 1785-1806.

DOI: 10.1042/BCJ20220244

Farrugia, C., Stafford, G.P., Gains, A.F., Cutts, A.R., Murdoch, C.

Fusobacterium nucleatum mediates endothelial damage and increased permeability following single species and polymicrobial infection

(2022) Journal of Periodontology, 93 (9), pp. 1421-1433.

DOI: 10.1002/JPER.21-0671

Lowry, R.C., Allihaybi, L., Parker, J.L., Couto, N.A.S., Stafford, G.P., Shaw, J.G.

Heterogeneous glycosylation and methylation of the Aeromonas caviae flagellin

(2022) MicrobiologyOpen, 11 (4), art. no. e1306, . DOI: 10.1002/mbo3.1306

Chinthamani, S., Settem, R.P., Honma, K., Stafford, G.P., Sharma, A.

Tannerella forsythia strains differentially induce interferon gamma-induced protein 10 (IP-10) expression in macrophages due to lipopolysaccharide heterogeneity

(2022) Pathogens and Disease, 80 (1), art. no. ftac008,

DOI: 10.1093/femspd/ftac008

Court EK, Chaudhuri RR, Kapoore RV, Villa RX, Pandhal J, Biggs CA and Stafford GP (2021) Looking through the FOG: microbiome characterization and lipolytic bacteria isolation from a fatberg site. Microbiology (Reading), December, 167(12), 001117.doi:https://doi.org/10.1099/mic.0.001117. PMID: 34870579; PMCID: PMC8744997.

Cheeseman J, Kuhnle G, Stafford G et al. (2021) Sialic acid as a potential biomarker for cardiovascular disease, diabetes and cancer. Biomarkers in Medicine, 2021, 15(11), pp. 911–928.

Flack EKP, Chidwick HS, Guchhait G, Stafford GP et al. (2020) Biocatalytic transfer of Pseudaminic Acid (Pse5Ac7Ac) using Promiscuous Sialyltransferases in a chemoenzymatic approach to Pse5Ac7Ac-containing glycosides. ACS Catalysis, 2020, 10(17), pp. 9986–9993.

Farrugia C, Stafford GP, Murdoch C (2020) Porphyromonas gingivalis outer membrane vesicles increase vascular permeability. Journal of Dental Research, December 2020, 99(13), pp. 1494–1501.doi: 10.1177/0022034520943187. Epub 2020 Jul 29. PMID: 32726180

Jakubovics NS, Goodman SD, Mashburn-Warren L, Stafford GP, Cieplik F (2020) The dental plaque biofilm matrix. Periodontol 2000, June 2021, 86(1), pp. 32–56.doi:https://doi.org/10.1111/prd.12361. Epub, 10 March 2021, PMID: 33690911.

Farrugia C, Stafford GP, Potempa J, Wilkinson RN, Chen Y, Murdoch C, Widziolek M (2020) Mechanisms of vascular damage by systemic dissemination of the oral pathogen Porphyromonas gingivalis. The FEBS Journal, March 2021, 288(5), pp. 1479–1495.doi:https://doi.org/10.1111/febs.15486. Epub 1 August 2020. PMID: 32681704.

Nash RJ, Bartholomew B, Penkova YB, Rotondo D, Yamasaka F, Stafford GP, Jenkinson SF, Fleet GWJ. (2020) Iminosugar idoBR1 Iiolated from cucumber cucumis sativus reduces inflammatory activity. ACS Omega. 23 Jun 2020, 5(26), pp. 16263-16271.doi:https://doi.org/10.1021/acsomega.0c02092. eCollection, 7 July 2020.

Ansbro K, Wade WG, Stafford GP (2020) Tannerella serpentiformis sp. nov., isolated from the human mouth. International Journal of Systematic and Evolutionary Microbiology, 70(6), pp.3749–3754.doi:https://doi.org/10.1099/ijsem.0.004229. Epub 2020 Jun 10.

Frey AM, Satur MJ, Phansopa C, Honma K, Urbanowicz PA, Spencer DIR, Pratten J, Bradshaw D, Sharma A, Stafford GP (2019) Characterization of Porphyromonas gingivalis sialidase and disruption of its role in host-pathogen interactions. Microbiology, 165(11), pp. 1181–1197.doi:https://doi.org/10.1099/mic.0.000851

Al-Zubidi M, Widziolek M, Court E, Gains A, Smith R, Ansbro K, Alrafaie A, Evans C, Murdoch C, Mesnage S, Douglas CWI, Rawlinson A, and Stafford GP (2019) Identification of novel bacteriophages with therapeutic potential targeting Enterococcus faecalis. Infection and Immunity, 87(11). 26 August 2019.pii: IAI.00512-19.doi:https://doi.org/10.1128/IAI.00512-19. [Epub ahead of print]

Green CA, Kamble NS, Court EK, Bryant OJ, Hicks MG, Lennon C, Fraser GM, Wright PC, Stafford GP (2019) Engineering the flagellar type III secretion system: improving capacity for secretion of recombinant protein. Microbial Cell Factories, 18, 10.doi:https://doi.org/10.1186/s12934-019-1058-4.

Frey AM, Satur MJ, Phansopa C, Parker JL, Bradshaw D, Pratten J and Stafford GP (2018) Evidence for a novel Carbohydrate Binding Module (CBM) of Tannerella forsythia NanH sialidase, key to interactions at the host-pathogen interface. Biochemistry Journal, 475,pp. 1159–1176.
Faculty of 1000 recommendation of this paper: https://f1000.com/prime/732763425?bd=1

Frey AM, Ansbro K, Kamble NS, Pham TK and Stafford GP (2018) Characterisation and pure culture of putative health-associated oral bacterium BU063 (Tannerella sp. HOT-286) reveals presence of a potentially novel glycosylated s-layer. FEMS Microbiology Letters, 365(17).doi:https://doi.org/10.1093/femsle/fny180.

Etxabide A, Ribeiro RDC, Guerrero P, Ferreira AM, Stafford GP, Dalgarno K, de la Caba K and Gentile P (2018) Lactose-crosslinked fish gelatin-based porous scaffolds embedded with tetrahydrocurcumin for cartilage regeneration. International Journal of Biological Macromolecules, 117, pp. 199–208.doi:https://doi.org/10.1016/j.ijbiomac.2018.05.154. Epub: 23 May 2018.

Govinden G, Parker JL, Naylor KL, Frey AM, Anumba DOC and Stafford GP (2018) Inhibition of sialidase activity and cellular invasion by the bacterial vaginosis pathogen Gardnerella vaginalis. Archives Microbiology, 200(7), pp. 1129–1133.doi:https://doi.org/10.1007/s00203-018-1520-4. Epub: 18 May 2018.

Wilcock CJ, Stafford GP, Miller CA, Ryabenkova Y, Fatima M, Gentile P, Möbus G and Hatton PV (2017) Preparation and antibacterial properties of silver-doped nanoscale hydroxyapatite pastes for bone repair and augmentation. Journal of Biomedical Nanotechnology, 13(9), pp. 1168–1176.doi:https://doi.org10.1166/jbn.2017.2387.

Stafford GP, Parker JL, Amabebe E, Kistler J, Reynolds S, Stern V, Paley M and Anumba DOC (2017) Spontaneous Preterm Birth Is Associated with Differential Expression of Vaginal Metabolites by Lactobacilli-Dominated Microflora. Frontiers in Physiol. 2017 Aug 23;8:615.doi:https://doi.org./10.3389/fphys.2017.00615. eCollection 2017.

Gul SS, Griffiths GS, Stafford GP, Al-Zubidi MI, Rawlinson A and Douglas CWI (2017) Investigation of a novel predictive biomarker profile for the outcome of Periodontal treatment. Journal of Periodontology, 88(11), pp. 1135–1144. doi:https://doi.org/10.1902/jop.2017.170187. Epub: 3 July 2017.

Amabebe E, Reynolds S, Stern VL, Parker JL, Stafford GP, Paley MN and Anumba DO (2016) Identifying metabolite markers for preterm birth in cervicovaginal fluid by magnetic resonance spectroscopy. Metabolomics, 2016, 12:67. Epub: 8 March 2016.

Thomson RI, Gardner R, Strohfeldt K, Fernandes D, Stafford GP, Spencer D and Osborn H (2016) Analysis of three epoetin alpha products by LC and LC-MS indicates differences in glycosylation critical quality attributes, including sialic acid content. Analytical Chemistry, 20, 89(12), pp. 6455–6462.

Stafford GP, Chaudhuri R, Haraszthy V, Friedrich V, Schäffer C, Ruscitto A, Honma K and Sharma A (2016) Draft genome sequences of three clinical isolates of Tannerella forsythia isolated from sub-gingival plaque from periodontitis patients in the USA. ASM Genome Announcements.

Widziolek M, Prajsnar TK, Tazzyman S, Stafford GP, Potempa J and Murdoch C (2016) Zebrafish as a new model to study effects of periodontal pathogens on cardiovascular diseases. Scientific Reports. Oct 25, 6:36023.

Ruscitto A, Hottmann I, Stafford GP, Schäffer C, Mayer C, Sharma A. (2016) Identification of a novel N-acetylmuramic acid (MurNAc) transporter in Tannerella forsythia. Journal of Bacteriology. 2016 Sep 6. pii: JB.00473-16.

Naylor KL, Widziolek M, Hunt S, Conolly M, Hicks M, Stafford GP, Potempa J, Murdoch C, Douglas CW, Stafford GP. (2016) Role of OmpA2 surface regions of Porphyromonas gingivalis in host-pathogen interactions with oral epithelial cells. MicrobiologyOpen, 6 September 2016.doi:https://doi.org/10.1002/mbo3.401.

Amabebe E, Reynolds S, Stern VL, Parker JL, Stafford GP, Paley MN, Anumba DO. (2016) Identifying metabolite markers for preterm birth in cervicovaginal fluid by magnetic resonance spectroscopy. Metabolomics, 12, 67.

Phansopa C, Kozak RP, Liew LP, Frey AM, Farmilo T, Parker JL, Kelly DJ, Emery RJ, Thomson RL, Royle L, Gardner RA, Spencer DI and Stafford GP (2015) Characterization of a sialate O-acetyl esterase (NanS) from the oral pathogen Tannerella forsythia that enhances sialic acid release by NanH its cognate sialidase. Biochemical Journal, 2015, 472(2), pp. 157–167.

Honma K, Ruscitto A, Frey AM, Stafford GP and Sharma A. (2015) Sialic acid transporter NanT participates in Tannerella forsythia biofilm formation and survival on epithelial cells. Microbial Pathogenesis, 28 August 2015. pii: S0882-4010(15)00143-6. doi:https://doi.org/10.1016/j.micpath.2015.08.012.

Gentile P, Frongia ME, Cardellach M, Miller CA, Stafford GP, Leggett GJ and Hatton PV (2015) Functionalised nanoscale coatings using layer-by-layer assembly for imparting antibacterial properties to polylactide-co-glycolide surfaces. Acta Biomaterialia, 2015, 21, pp. 35–43.

Megson ZA, Koerdt A, Scuster H, Ludwig R, Janesch B, Frey A, Naylor K, Wilson IB, Stafford GP, Messner P and Schaeffer C (2015) Characterisation of an alpha-L-fucosidase from the periodontal pathogen Tannerella forsythia. Virulence, 2015, 6, pp. 282–292.

Lowry RC, Parker JL, Kumbhar R, Mesnage S, Shaw JG and Stafford GP (2014) The Aeromonas caviae AHA0618 gene modulates cell length and influences swimming and swarming motility. Microbiology Open, Article first published online: 17 DEC 2014.doi:https://doi.org/10.1002/mbo3.233.

Parker JL, Lowry RC, Couto NA, Wright PC, Stafford GP, Shaw JG. (2014) Maf-dependent bacterial flagellin glycosylation occurs before chaperone binding and flagellar T3SS export. Molecular Microbiology, April 2014, 92(2), pp. 258–72.

Phansopa C, Roy S, Rafferty JB, Douglas CWI, Pandhal J, Wright PC, Kelly DJ and Stafford GP (2013) Structural and functional characterisation of NanU, a novel high-affinity sialic acid-inducible binding protein of oral and gut-dwelling Bacteroidetes spp. Biochemical Journal, 458(3), pp.499–511.

Stafford P, Higham J, Pinnock A, Murdoch C, Douglas CWI, Stafford GP & Lambert DW (2013) Gingipain-dependent degradation of mTOR pathway proteins by the periodontal pathogen Porphyromonas gingivalis during invasion. Molecular Oral Microbiology, 27, pp.11–22.

Settem RP, Honma K, Nakajima T, Phansopa C, Roy S, Stafford GP and Sharma A (2012) A bacterial glycan core linked to surface (S)-layer proteins modulates host immunity through Th17 suppression. Mucosal Immunology, 6, pp. 415–426.

Settem RJ, El-Hassan AT, Honma K, Stafford GP and Sharma A (2012) Fusobacterium nucleatum and Tannerella forsythia induce synergistic alveolar bone loss in a mouse periodontitis model. Infection and Immunity, 80(7), pp.2436–2443.

Parker JL, Day-Williams MJ, Tomas JM, Stafford GP and Shaw JG (2012) Identification of a putative glycosyltransferase responsible for the transfer of pseudaminic acid onto the polar flagellin of Aeromonas caviae Sch3N. MicrobiologyOpen, 1(2), pp. 149–160.

Roy S, Phansopa C, Stafford P, Honma K, Douglas I, Sharma A and Stafford GP (2012) Beta-hexosaminidase activity of the oral pathogen Tannerella forsythia influencesbiofilm formation on glycoprotein substrates. FEMS Immunology and Medical Microbiology, 65(1), pp. 116–20.

Roy S, Honma K, Douglas CWI, Sharma A and Stafford GP (2011) Role of sialidase in glycoprotein utiisation by Tannerella forsythia. Microbiology, 157, pp. 3195–3202.  

Roy S, Douglas CI and Stafford GP (2010) A novel sialic acid utilisation and uptake system in the periodontal pathogen Tannerella forsythia. Journal of Bacteriology, 2010, 192, pp. 2285–2293.  

Pham TK, Roy S, Douglas CWI, Wright PC and Stafford GP (2010) A quantitative analysis of biofilm adaptation by the periodontal pathogen Tannerella forsythia. Proteomics, 10, pp. 3130–3141 

Suwannakul S, Stafford GP, Wan Sulaiman WN, Whawell SA and Douglas CWI (2009) Identification of bistable populations of Porphyromonas gingivalis that differ in epithelial cell invasion. Microbiology, 156, pp. 3052–3064. 

Stafford GP, Evans LDB, Krumscheid R, Dhillon P, Fraser GM and Hughes C (2007) Sorting of early and late flagellar subunits after docking at the membrane ATPase of the type III export pathway. Journal of Molecular Biology, 374, pp. 877–882.

Stafford GP and Hughes C (2007) Salmonella typhimurium flhE, a conserved flagellar regulon gene required for swarming. Microbiology, 153, 541–547.

Evans LD, Stafford GP, Ahmed S, Fraser GM and Hughes C (2006) An escort mechanism for cycling of export chaperones during flagellum assembly. Proceedings of the National Academy of Sciences USA, 103, pp. 17474–17479.  

Stafford GP, Ogi T and Hughes, C (2005) Binding and transcriptional activation of non-flagellar genes by the E. coli flagella master regulator FlhD2C2. Microbiology, 151, pp. 1779-1788.   

Thomas J, Stafford GP and Hughes C (2004) Docking of cytosolic chaperone-substrate complexes at the membrane ATPase during flagellar type III protein export. Proceedings of the National Academy of Sciences USA, 101, 3945-50.  

Stafford GP, Scanlan J, McDonald IR and Murrell JC (2003) rpoN, mmoR and mmoG, genes involved in regulating the expression of soluble methane monooxygenase in Methylosinus trichosporium OB3b. Microbiology, 149, pp. 1771-84.  

Karlsen OA, Berven FS, Stafford GP, Larsen Ø, Murrell JC, Jensen HB and Fjellbirkeland A. (2003) The surface-associated and secreted MopE protein of Methylococcus capsulatus (Bath) responds to changes in the concentration of copper in the growth medium. Applied and Environmental Microbiology, 69(4), pp. 2386-2388.               

Gilbert B, McDonald IR, Finch R, Stafford GP, Nielsen AK, Murrell JC. (2000) Molecular analysis of the pmo (particulate methane monooxygenase) operons from two type II methanotrophs. Applied and Environmental Microbiology, 66(3), pp. 966-75.     

Cook GM, Loder C, Søballe B, Stafford GP, Membrillo-Hernández J, Poole RK. (1998) A factor produced by Escherichia coli K-12 inhibits the growth of E. coli mutantsdefective in the cytochrome bd quinol oxidase complex: enterochelin rediscovered. Microbiology. 144 :3297-308.  

Invited review:
Douglas CW, Naylor K, Phansopa C, Frey AM, Farmilo T and Stafford GP (2014) Physiological adaptations of key oral bacteria. Advances in Microbial Physiology, 2014, 65, pp. 257–335.              

Stafford GP, Roy S, Honma K and Sharma A (2012) Sialic acid, periodontal pathogens and Tannerella forsythia: stick around and enjoy the feast! Molecular Oral Microbiology, 27, pp. 11–22.

Settem RP, Honma K, Stafford GP, Sharma A. (2013) Protein-linked glycans in periodontal bacteria: prevalence and role at the immune interface. Front Microbiology, 17, 4:310.